課程名稱 |
質譜儀及其應用 MASS SPECTROMETRY AND ITS APPLICATION |
開課學期 |
97-2 |
授課對象 |
理學院 化學所化學生物學組 |
授課教師 |
陳仲瑄 |
課號 |
Chem5054 |
課程識別碼 |
223 U2510 |
班次 |
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學分 |
3 |
全/半年 |
半年 |
必/選修 |
選修 |
上課時間 |
星期五6,7,8(13:20~16:20) |
上課地點 |
新401 |
備註 |
本課程中文授課,使用英文教科書。外系所學生選修需經授課教師同意。 限學士班三年級以上 且 限本系所學生(含輔系、雙修生) 總人數上限:60人 |
Ceiba 課程網頁 |
http://ceiba.ntu.edu.tw/972mass |
課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
課程大綱
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課程概述 |
I. Introduction:
I.a Basic of Mass Spectrometry
I.b Major Applications of Mass Spectrometry
I.c Vacuum Science and Technology
II. Ionization:
II.a Electron Ionization
II.b Chemical Ionization
II.c Photoionization
II.d Matrix-assisted Laser Desorption/Ionization
II.e Electrospray Ionization
II.f Other Ionization methods: FAB, Field Ionization, SIMS etc.
II.g Major challenges for Future Development
III. Mass Analysis:
III.a Quadrupole
III.b Ion Trap
III.c Time-of-flight
III.d Magnetic Sector
III.e FTICR
III.f Major Challenges for Future Development
IV. Detection:
IV.a Charge Collector
IV.b Charge Amplifier Detector
IV.c Scientillator Detector
IV.d Other Detectors and Major Barriers to Overome.
V. Tandem Mass Spectrometry
V.a Basic of MS/MS
V.b Collision induced Dissociation
V.c Ion-Molecule Interaction
VI. Chromatography/Mass Spectrometer Coupling
VI.a GC/MS
VI.b LC/MS
VI.c CE/MS
VII Biomolecule Analysis
VII.a Oligonucleotide, Protein, Polysaccharide Analysis
VII.b DNA Analysis and Sequencing
VII.c Proteomic & Metabolomic Applications
VII.d Disease Diagnosis
VIII Other Applications
VIII.a Trace Element Analysis
VIII.b Organic Polymer Analysis
VIII.c Environmental Application
VIII.d Material Analysis
IX Future Challenges:
I X.a Ionization Mechanism
IX.b Mass to Charge Ratio Extension
IX.c Quantitative Measurements
IX.d Dynamic Range Extension
IX.e Cost Reduction
IX.f Size Reduction
X. Conclusion
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課程目標 |
I. Introduction:
I.a Basic of Mass Spectrometry
I.b Major Applications of Mass Spectrometry
I.c Vacuum Science and Technology
II. Ionization:
II.a Electron Ionization
II.b Chemical Ionization
II.c Photoionization
II.d Matrix-assisted Laser Desorption/Ionization
II.e Electrospray Ionization
II.f Other Ionization methods: FAB, Field Ionization, SIMS etc.
II.g Major challenges for Future Development
III. Mass Analysis:
III.a Quadrupole
III.b Ion Trap
III.c Time-of-flight
III.d Magnetic Sector
III.e FTICR
III.f Major Challenges for Future Development
IV. Detection:
IV.a Charge Collector
IV.b Charge Amplifier Detector
IV.c Scientillator Detector
IV.d Other Detectors and Major Barriers to Overome.
V. Tandem Mass Spectrometry
V.a Basic of MS/MS
V.b Collision induced Dissociation
V.c Ion-Molecule Interaction
VI. Chromatography/Mass Spectrometer Coupling
VI.a GC/MS
VI.b LC/MS
VI.c CE/MS
VII Biomolecule Analysis
VII.a Oligonucleotide, Protein, Polysaccharide Analysis
VII.b DNA Analysis and Sequencing
VII.c Proteomic & Metabolomic Applications
VII.d Disease Diagnosis
VIII Other Applications
VIII.a Trace Element Analysis
VIII.b Organic Polymer Analysis
VIII.c Environmental Application
VIII.d Material Analysis
IX Future Challenges:
I X.a Ionization Mechanism
IX.b Mass to Charge Ratio Extension
IX.c Quantitative Measurements
IX.d Dynamic Range Extension
IX.e Cost Reduction
IX.f Size Reduction
X. Conclusion
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
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評量方式 (僅供參考) |
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週次 |
日期 |
單元主題 |
第12週 |
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質譜儀及其應用報告的題目 |
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